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Hybrid-R™ miRNA

In recent years, interest in small RNA, such as siRNA and miRNA which are related to research of gene regulation, has expanded. There are many commercial kits for total RNA preparation, but most of these are focused on preparation of large RNA longer than 200 nucleotides. Because both siRNA and miRNA are between 15 ~ 30 nucleotides in length, the need of specially optimized kit for small RNA (< 200 nucleotides) is growing rapidly. GeneAll® Hybrid-R™ miRNA is designed for purification of large and small RNA separately from culture cells or animal tissues and co-purification in a single tube is also available by modified protocol.

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Overview

In recent years, interest in small RNA, such as siRNA and miRNA which are related to research of gene regulation, has expanded. 

There are many commercial kits for total RNA preparation, but most of these are focused on preparation of large RNA longer than 200 nucleotides. 

Because both siRNA and miRNA are between 15 ~ 30 nucleotides in length, the need of specially optimized kit 

for small RNA (< 200 nucleotides) is growing rapidly. Hybrid-R™ miRNA is designed for purification of large and small RNA separately 

from culture cells or animal tissues and co-purification in a single tube is also available by modified protocol. 

01

Format

Hybrid-R™ miRNA utilizes the lysis method of RiboExTM which has a powerful ability of lysis and the purification method based on glassfiber membrane technology. 

  • Combined Sample lysis by RiboExTM with Spin column format
02

Stable and consistent yield

  • Recovery range
    : Large RNA : >200 nucleotides
      Small RNA : <200 nucleotides
03

Simple and safe procedure

  • Preparation time : ~30 minutes
  • No ethanol precipitation
04

Applications

  • Suitable for the isolation of Poly A+ RNA,northern blotting, dot blotting, in vitro translation, cloning, RT-PCR, RPA and other analytical procedures.

specifications

Technical Information

Features Hybrid-R™ miRNA
Format Column Type B with collection tube
Column Type W with collection tube
Maximum amount of starting samples ~100 mg tissue or ~1 x 107 cells
Maximum loading volume ~700 μl
Minimum elution volume ~30 μl
Maximum Binding capacity ~100 μg

Kit Contents

Hybrid-R™ miRNA (Cat. No. 325-150)
  • RIboExTM
  • Column type B 
  • Column type W 
  • Collection tubes
  • Buffer SW1
  • Buffer RBW (concentrate)
  • Buffer RNW (concentrate)
  • Nuclease-free water
    		•

    Technical Data

        Real-Time PCR amplification of miR-24 from Small RNA

           

        Extraction of Large and small RNA

           

        Comparison Data - miRNA Extraction 

           

        Large RNA Purification and cDNA synthesis.