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HyperScript™ One-Step RT-PCR Master Mix

HyperScript™One-Step RT-PCR Master Mix is a 2X premix ready to use for Reverse Transcriptase(RT) reaction and Polymerase Chain Reaction(PCR). This mix contains HyperScript™ reverse transcriptase and AmpONE™ HS-Taq DNA polymerase and both RT and PCR reactions are carried out successively in a single tube. HS-Taq polymerase can amplify the fragment up to 5 kb in length.

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Overview

HyperScript™ One-Step RT-PCR Master mix is a 2X premix ready to use for Reverse Transcriptase(RT) reaction and Polymerase Chain Reaction(PCR). 

This mix contains HyperScript™ reverse transcriptase and AmpONE™ HS-Taq DNA polymerase and 

both RT and PCR reactions are carried out successively in a single tube. HS-Taq polymerase can amplify the fragment up to 5 kb in length.

01

Description

  • Pre-mixed solution for cDNA synthesis and PCR amplification.
02

Synthesis efficiency

  • Extension up to 5 kb
  • Maximize the thermal stability
    by using Hot start Taq
03

Easy to use

  • 2X solution type
    : Contains all reaction components
04

Applications

  • Suitable for cDNA synthesis and gene amplification by PCR in a single tube.

specifications

Technical Information

Features Specifications
Unit definition One unit incorporates 1 nmol of dTTP into acid-precipitable material in 10 min at 37°C using poly(A) · oligo(dT)20 as template- primer.
Synthesis efficiency ~5 kb
Storage condition Stable for 1 year at -20°C

Kit Contents

HyperScript™ One-Step RT-PCR Master Mix (Cat. No. 602-110)
  • HyperScript™  One-Step RT-PCR Master Mix (2X) (500 μl x 2 tube)
    contains HyperScript™ reverse transcriptase, HS-Taq DNA polymerase, reaction buffer, dNTPs, RNase inhibitor and stabilizer.

  • Not contain primers and templates
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