GeneAll

APPLICATIONS

AGRICULTURE, PLANT AND FISHERY

07
Fishery:
Halophyte DNA
In nature, there are many plants that live in unique environments that are different from the general environment. Among them, halophytes (e.g., Spergularia marina, Suaeda japonica, and Suaeda maritima) are plants that live in environments with salt, such as coastal wetlands, sand dunes, and salt pans. The criteria for classifying halophytes vary among taxonomist, such as whether they require salt, their habitat, or their physiological characteristics. They are mainly used as edible or medicinal resources in the food or pharmaceutical fields. In genetic research, accumulated salt and polysaccharides in halophytes are among the factors that significantly reduce nucleic acid extraction efficiency, making them one of the difficult materials to handle.
Exgene™ Plant SV is a product for genomic DNA extraction from various plant samples, and customers are able to choose mini, midi, and maxi scale following sample amount. This product is specialized for high-quality DNA extraction using EzSep™ Filter for efficient removing of polysaccharide and cell debris that reduce nucleic acid extraction efficiency in plants, and the purified DNA can be applied to various downstream applications.
The following study analyzes the genomic DNA extracted from Spergularia marina, type of halophyte, using Exgene™ Plant SV maxi based on yield, purity, and gel electrophoresis. In this experiment, we applied a customized protocol to remove the large amount of salt and polysaccharide in halophytes that lower the nucleic acid extraction efficiency. Also, we compared the yield and purity with those obtained by extracting DNA using standard protocol.
06
Plant:
Leaf DNA
Plants are known to be the most species distributed on Earth, and this diversity is most closely related to plant research. The plant research is difficult due to the specificity of each plant species especially in the field of molecular biology. Debris removal is essential at the nucleic acid extraction stage, and it is most important to remove it effectively. This application note is data obtained from high-quality gDNA from various plant leaves using the Exgene™ Plant SV mini kit, and please use it as a reference for plant research.
05
Agriculture:
Fruit RNA
Damage to food resources is accelerating due to various effects such as pests that hinder plant growth. Research to improve varieties and produce healthy crops to prevent such damage and to produce eco-friendly crops is becoming important.This application note extracts total RNA with Ribospin™ Seed/Fruit kit from pulp samples of various fruits/fruits, and please use it as a reference for research.
04
Agriculture:
Seed RNA
The vitality of crops is largely controlled by various environmental factors (temperature, precipitation, sunlight, etc.). These environmental factors cannot be artificially controlled, so crop improvement is essential according to environmental changes. This application note is the result of extracting total RNA from various plant seed samples with Ribospin™ Seed/Fruit kit. Please use it as a reference for seed research.
03
Plant:
Leaf RNA
As climate change accelerates, the plant research is becoming more active to preserve food resources. The extraction of highly concentrated /purified nucleic acids is essential for the research. This application note is the result of extracting total RNA from rice and potato leaf samples with Ribospin™ Seed/Fruit kit, and please use it as a reference for plant research.
02
Fishery:
Sea algae RNA
Since sea algae is the primary producers of the marine ecosystem and is widely used as human edible material or bio-resource, many studies are being conducted. However, it is known that nucleic acid extraction is difficult because the salt contained in sea algae acts as an obstacle to extracting nucleic acids.
This application note is an experiment to confirm whether it is possible to extract total RNA from sea algae samples with Ribospin™ Plant kit, and please use it as a reference for sea algae research.
01
Fishery:
Sea algae DNA
Sea algae plays as a primary producer in the marine eco system and is widely used as nutritional and functional food sources or bio-resource. So many studies have been conducted, but salt contained in sea algae is known to hinder nucleic acid extraction, making it difficult to extract nucleic acids.This application note is the result of checking whether gDNA can be extracted from the seaweed sample with Exgene™ Plant SV mini kit, and please use it as a reference for seaweed research.