- 07
Fishery:
Halophyte DNA - In nature, there are many plants that live in unique environments that are different from the general environment. Among them, halophytes (e.g., Spergularia marina, Suaeda japonica, and Suaeda maritima) are plants that live in environments with salt, such as coastal wetlands, sand dunes, and salt pans. The criteria for classifying halophytes vary among taxonomist, such as whether they require salt, their habitat, or their physiological characteristics. They are mainly used as edible or medicinal resources in the food or pharmaceutical fields. In genetic research, accumulated salt and polysaccharides in halophytes are among the factors that significantly reduce nucleic acid extraction efficiency, making them one of the difficult materials to handle.
Exgene™ Plant SV is a product for genomic DNA extraction from various plant samples, and customers are able to choose mini, midi, and maxi scale following sample amount. This product is specialized for high-quality DNA extraction using EzSep™ Filter for efficient removing of polysaccharide and cell debris that reduce nucleic acid extraction efficiency in plants, and the purified DNA can be applied to various downstream applications.
The following study analyzes the genomic DNA extracted from Spergularia marina, type of halophyte, using Exgene™ Plant SV maxi based on yield, purity, and gel electrophoresis. In this experiment, we applied a customized protocol to remove the large amount of salt and polysaccharide in halophytes that lower the nucleic acid extraction efficiency. Also, we compared the yield and purity with those obtained by extracting DNA using standard protocol.