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Exgene™ Plant SV

GeneAll® Exgene™ Plant SV provides a simple and easy method for the small, medium and large scale purification of total DNA from various plant tissues. With EzSep™ Filter and Column Type G, the procedure can be done in just 40 minutes (mini), yielding a pure genomic DNA suitable for various downstream appilcations without further manipulation.

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Overview

GeneAll® Exgene™ Plant SV provides a simple and easy method for the small, medium and large scale purification of total DNA 

from various plant tissues. With EzSep™ Filter and Column Type G, the procedure can be done in just 40 minutes (mini), 

yielding a pure genomic DNA suitable for various downstream appilcations without further manipulation.

01

Format

Exgene™ Plant SV utilizes glass microfiber membrane.

  • Spin column format
  • Midi and MAXI kits require the centrifuge which has swing bucket rotor and ability of 4,000 x g at least.
02

High purity

  • Stable and consistent result
  • Perfect removal of second metabolites such as polyphenols and polysaccharides
03

Simple and safe procedure

  • Simple procedure by the use of EzSepTM Filter
  • No phenol/chloroform extractions
  • No alcohol precipitation
04

Applications

  • Ready for use in PCR, Southern blotting, AFLP, RFLP, RAPD and other enzymatic reactions.

specifications

Technical Information

Features Plant SV mini Plant SV Midi Plant SV MAXI
Format Column type G (mini) with collection tubeColumn type G (Midi) with collection tubeColumn type G (MAXI) with collection tube
Maximum sample size
 ~100 mg wet
(25 mg dry)
~400 mg wet
(100 mg dry)
~1 g wet
(250 mg dry)
Preparation time<40 minutes

<60 minutes 

<60 minutes 

Maximum loading volume750 μl

5 ml

15 ml

Typical yield4~40 μg10~150 μg40~300 μg
Elution volume30~400 μl200~600 μl0.4~2 ml

Kit Contents

Plant SV mini

(Cat. No. 117-101 / 117-152)

Plant SV Midi

(Cat. No. 117-226 / 117-201)

Plant SV MAXI

(Cat. No. 117-310 / 117-326)

  • mini column type G
  • EzSepTM Filter
  • Collection tubes
  • Buffer PL
  • Buffer PD
  • Buffer BD (concentrate)
  • Buffer CW (concentrate)
  • Buffer AE
  • RNase A (100 mg/ml)
  • Midi column type G
  • EzSepTM Filter
  • Collection tubes
  • Buffer PL
  • Buffer PD
  • Buffer BD (concentrate)
  • Buffer CW (concentrate)
  • Buffer AE
  • RNase A (100 mg/ml)
  • MAXI column type G
  • EzSepTM Filter
  • Collection tubes
  • Buffer PL
  • Buffer PD
  • Buffer BD (concentrate)
  • Buffer CW (concentrate)
  • Buffer AE
  • RNase A (100 mg/ml)
  • Technical Data

        Comparison of Genomic DNA Extraction

               
     

     

        Compatibility Test with Restriction Enzymes

               
     

     

        PCR Amplification Test

               

    Protocols

    Supplementary Protocols

    Resources