GeneAll

PRODUCTS

이미지 이미지
RUO

AmpMaster™ 2X Taq Master Mix

AmpMaster™ 2X Taq Master Mix is purified from the cloned Thermus aquaticus DNA polymerase gene in Escherichia coli (E.coli). This master mix contains all reaction components required for conventional PCR, such as reaction buffer, dNTP, loading dye, stabilizer and sediment in addition to Taq DNA polymerase. It is recommended for use in conventional PCR (below 5 kb), TA cloning and colony PCR. It is ready-to-use mixture pipetting steps are minimized, reducing the possibility of errors and contamination.

SCALE / SIZE

Overview

AmpMaster™ 2X Taq Master Mix is purified from the cloned Thermus aquaticus DNA polymerase gene in Escherichia coli (E.coli). This master mix contains all reaction components required for conventional PCR, such as reaction buffer, dNTP, loading dye, stabilizer and sediment in addition to Taq DNA polymerase. It is recommended for use in conventional PCR (below 5 kb), TA cloning and colony PCR. It is ready-to-use mixture pipetting steps are minimized, reducing the possibility of errors and contamination. 

01

Description

  • This master mix contains all reaction components required for conventional PCR, such as reaction buffer, dNTP, loading dye, stabilizer and sediment in addition to Taq DNA polymerase.
02

Ready to use

  • 2X solution type
  • Suitable for the amplification of fragments up to 5 kb from template.
03

3' end A-tailing

  • 5’→3’ polymerase activity
  • No 3’→5’ exonuclease (proofreading) activity
04

Applications

  • Suitable for Conventional PCR (up to 5 kb), TA cloning, Colony PCR, High through-put PCR, Routine PCR requiring high reproducibility, DNA sequencing template preparation.

specifications

Technical Information

Features Specifications
Synthesis efficiency ~5 kb
Exonuclease activity None detected
Endonuclease activity None detected
Storage condition Stable for 18 months at -20°C

Kit Contents

Cat. No. 541-010

Cat. No. 541-050

  • 2X Taq Master Mix with Dye
  • (0.5 ml x 2 tubes)
  • 2X Taq Master Mix with Dye
  • (0.5 ml x 10 tubes)
  • Technical Data

    Protocols

    Supplementary Protocols