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AmpONE™ Taq DNA Polymerase

AmpONE™ Taq DNA Polymerase is olymerase is a highly purified recombinant enzyme that expresses the Thermus aquaticus DNA polymerase gene in Escherichia coli (E.coli) and is used for conventional PCR, DNA sequencing, TA cloning, and etc.

SCALE / SIZE

Overview

AmpONE™ Taq DNA Polymerase is olymerase is a highly purified recombinant enzyme that expresses the Thermus aquaticus DNA polymerase gene in Escherichia coli (E.coli) and is used for conventional PCR, DNA sequencing, TA cloning, and etc. 

01

Description

  • Recombinant enzyme derived from Thermus aquaticus, which is cloned and expressed in Escherichia coli and possesses the same functions as the native enzyme.
02

High purity​

  • No contamination of genomic DNA
  • Suitable for the amplification of fragments up to 5 kb from template.
03

3' end A-tailing

  • 5’→3’ polymerase activity
  • No 3’→5’ exonuclease (proofreading) activity
04

Applications

  • Suitable for various experiments such as conventional PCR, DNA sequencing and TA cloning.

specifications

Technical Information

Features Specifications
Unit definition One unit is the amount of AmpONE™ Taq DNA Polymerase required to incorporate 10 nmol of dNTP into acid-insoluble product in 30 minutes at 72°C.
Synthesis efficiency ~5 kb
Exonuclease activity None detected
Endonuclease activity None detected
Storage condition Stable for 18 months at -20°C

Kit Contents

Cat. No. 501-025

(250 units)

Cat. No. 501-050

(500 units)

Cat. No. 501-100

(1,000 units)
Taq DNA polymerase 50 μl x 1 tube
 50 μl x 2 tubes 50 μl x 4 tubes
10X Reaction Buffer
 1.0 ml x 1 tube 1.0 ml x 2 tubes
 1.0 ml x 4 tubes
2.5 mM dNTP Mix 0.8 ml x 1 tube
 0.8 ml x 2 tubes
 0.8 ml x 4 tubes
6X Loading Dye 1.0 ml x 2 tubes
 1.0 ml x 4 tubes
 1.0 ml x 8 tubes

Technical Data

Protocols

Handbooks

Supplementary Protocols