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Expin™ PCR SV

GeneAll® Expin™ PCR SV provides fastest and easiest method for reliable purification of DNA from PCR products or other enzymatic reaction mixtures without agarose gel electrophoresis. In this kit, glass fiber membrane is used to recover DNA of 100 bp to 10 kb, which is free of primer dimers, nucleotides, enzymes and salts in yields reaching 95%.

SCALE / SIZE

Overview

GeneAll® Expin™ PCR SV provides fastest and easiest method for reliable purification of DNA from PCR products or 

other enzymatic reaction mixtures without agarose gel electrophoresis. In this kit, glass fiber membrane is used to 

recover DNA of 100 bp to 10 kb, which is free of primer dimers, nucleotides, enzymes and salts in yields reaching 95%.

01

Format

Expin™ PCR SV utilizes glassfiber membrane technology to recover DNA from PCR products or other enzymatic reaction mixtures without agarose gel electrophoresis.

  • Spin column format
02

Stable and consistent yield

  • High recovery rate
    : 90~95% (100 bp~10 kb)
  • High purity
    : A260/A280 = 1.8~2.0
03

Simple and safe procedure

  • Preparation time : ~6 minutes
  • No use of organic solvents
  • No alcohol precipitation
04

Applications

  • Applied directly in ligation, automated sequencing, restriction enzyme assay, PCR, in vitro transcription, hybridization, microarray assay and other enzymatic reactions.

specifications

Technical Information

Features Expin™  PCR SV 
Format Column Type D with collection tube
Standard sample size 100 μl of PCR or enzymatic reaction mixtures
Recovered DNA size 100 bp~10 kb
Recovery rate
≤95%
Maximum loading volume of spin column ~750 μl
Minimum elution volume ~30 μl
Binding capacity ~10 μg

Kit Contents

Expin™ PCR SV (Cat. No. 103-150 / 103-102)

  • Column type D 
  • Buffer PB
  • Buffer NW
  • Buffer EB
  • Technical Data

        Extraction Efficiency

           
           

    Protocols

    Supplementary Protocols