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Expin™ CleanUp SV

GeneAll® Expin™ CleanUp SV is designed for fast and simple method for purification of fragment DNA of 40 bp to 10 kb from various enzymatic reactions in just 6 minutes. Purified DNA with this kit is free of nucleotides, enzymes and salts in yields reaching 95%, and is ready for automated sequencing, cloning, in vitro transcription, microarray and other enzymatic reactions.

SCALE / SIZE

Overview

GeneAll® Expin™ CleanUp SV is designed for fast and simple method for purification of fragment DNA of 40 bp to 10 kb 

from various enzymatic reactions in just 6 minutes. Purified DNA with this kit is free of nucleotides, enzymes and salts in yields reaching 95%, 

and is ready for automated sequencing, cloning, in vitro transcription, microarray and other enzymatic reactions.

01

Format

Expin™ CleanUp SV utilizes glassfiber membrane technology to recover DNA from various enzymatic reaction mixtures.

  • Spin column format
02

Stable and consistent yield

  • High recovery rate
    : 80~95% (40 bp~10 kb)
  • High purity
    : A260/A280 = 1.8~2.0
03

Simple and safe procedure

  • Preparation time : ~6 minutes
  • No use of organic solvents
  • No alcohol precipitation
04

Applications

  • Applied directly in ligation, automated sequencing, restriction enzyme assay, PCR, in vitro transcription, hybridization, microarray assay and other enzymatic reactions.

specifications

Technical Information

Features Expin™  CleanUp SV 
Format Column Type D with collection tube
Standard sample size 50 μl of enzyme mixtures
Recovered DNA size 40 bp~10 Kb
Recovery rate
≤95%
Maximum loading volume of spin column ~750 μl
Minimum elution volume ~30 μl
Binding capacity ~10 μg

Kit Contents

Expin™ CleanUp SV (Cat. No. 113-150 / 113-102)  

  • Column type D 
  • Buffer NR
  • Buffer NW
  • Buffer EB
  • Technical Data

        Consistent Result from Various Size

           

        DNA Automated Sequencing Analysis

           

    Protocols

    Supplementary Protocols