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Ribospin™

GeneAll® Ribospin™ provides a convenient method for isolation of total RNA from cell and tissue samples. Ribospin™ procedures employed the glassfiber membrane technology for the fastest and the most convenient of high purity RNA isolation, instead of conventional alcohol precipitation or phenol / chloroform extraction. Whole procedure takes only 15 minutes and the eluates are suitable for RT-PCR or any downstream application without further manipulation.

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Overview

GeneAll® Ribospin™ provides a convenient method for isolation of total RNA from cell and tissue samples. 

Ribospin™ procedures employed the glassfiber membrane technology for the fastest and the most convenient of high purity RNA isolation,

 instead of conventional alcohol precipitation or phenol / chloroform extraction. Whole procedure takes only 15 minutes 

and the eluates are suitable for RT-PCR or any downstream application without further manipulation.

01

Format

Ribospin™ utilizes glassfiber membrane technology for the isolation of total RNA.

  • Spin column format
02

Stable and consistent yield

  • Typical yield
    : ~20 μg / 1 x 106 cultured cells,
      ~60 μg / 10 mg liver tissue
  • High purity : A260/A230 > 2.0, A260/A280 > 1.8
03

Simple and safe procedure

  • Preparation time : ~15 minutes
  • No phenol/chloroform extraction
  • No alcohol precipitation
04

Applications

  • Ready for use in RT-PCR, northern blotting, dot blotting, in vitro translation, molecular cloning, real-time PCR, RNase protection assays and other analytical procedures

specifications

Technical Information

Features Ribospin™ 
Format Column Type F with collection tube
Maximum amount of starting samples ~25 mg tissue or ~5 x 106 cells
Maximum loading volume ~700 μl
Minimum elution volume ~40 μl
Binding capacity ~100 μg

Kit Contents

Ribospin™ (Cat. No. 304-150)

  • Column type F 
  • Collection tubes
  • Buffer LYS
  • Buffer GW1
  • Buffer RNW (concentrate)
  • Nuclease-free water
  • Technical Data

        The Yield of Total RNA from Various Starting Materials Using RibospinTM

                      

     

        Comparison Data

           
           

    Protocols

    Supplementary Protocols