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Allspin™

GeneAll® Allspin™ total DNA / RNA purification kit provides a convenient method for the isolation of total DNA and total RNA simultaneously from a single sample of tissue or cultured cells. DNA and RNA are purified separately from a same sample by individual but successive procedure using column B and column W respectively. Whole procedure can be performed in just 30 minutes and the length of obtained DNA is up to 50 kb (average is 30 kb) and that of RNA is longer than 200 nucleotides.

SCALE / SIZE

Overview

GeneAll® Allspin™ total DNA / RNA purification kit provides a convenient method for the isolation of total DNA and total RNA simultaneously 

from a single sample of tissue or cultured cells. DNA and RNA are purified separately from a same sample by individual

but successive procedure using column B and column W respectively. Whole procedure can be performed in just 30 minutes and

the length of obtained DNA is up to 50 kb (average is 30 kb) and that of RNA is longer than 200 nucleotides.  

01

Format

Allspin™ utilizes glassfiber membrane technology for the isolation of total DNA and total RNA.

  • Spin column format
02

Stable and consistent yield

  • Typical yield of RNA
    : ~20 μg / 1 x 106cultured cells,
        60 μg / 10 mg liver tissue
  • Typical yield of DNA
    : ~10 μg / 1 x 106 cultured cells,
      ~25 μg / 10 mg liver tissue
03

Simple and safe procedure

  • Preparation time : ~30 minutes
  • No phenol/chloroform extraction
  • No alcohol precipitation
04

Applications

  • Ready for use in RT-PCR, northern blotting, dot blotting, in vitro translation, molecular cloning, real-time PCR, RNase protection assays and other analytical procedures

specifications

Technical Information

Features Allspin™ for DNA  Allspin™ for RNA
Format Column Type B with collection tubeColumn Type W with collection tube
Maximum amount of starting samples ~30 mg tissue or ~1 x 107 cells~30 mg tissue or ~1 x 107 cells
Maximum loading volume ~750 μl ~750 μl
Minimum elution volume ~50 μl ~30 μl
Binding capacity ~100 μg ~100 μg
Nucleic acid binding size ~50 Kb>200 nucleotides

Kit Contents

Allspin™ (Cat. No. 306-150)

  • Column type B (Red ring)
  • Column type W (Blue ring)
  • Collection tubes
  • Buffer CTL
  • Buffer GW1
  • Buffer BW (concentrate)
  • Buffer RNW (concentrate)
  • Buffer AE
  • Technical Data

        The Yield of Genomic DNA and Total RNA from Various Starting Materials Using AllspinTM

               

        Comparison Data

           


           

    Protocols

    Supplementary Protocols