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RealAmp™ 2X qPCR Master Mix

RealAmp™ 2X qPCR Master Mix provides fast and robust amplification across a wide range of templates through high quality antibody-based hot start PCR enzyme without compromising sensitivity, specificity, dynamic range, or PCR efficiency. GeneAll has engineered most optimal reaction buffers to minimize non-specific amplification and / or primer dimer formation. RealAmp™ 2X qPCR Master mix, supplied in a 2X concentration, is a ready-to-use master mix to perform real-time PCR using dsDNA binding fluorescent dye.

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Overview

GeneAll® RealAmp™ 2X qPCR Master Mix provides fast and robust amplification across a wide range of templates through high quality 

antibody-based hot start PCR enzyme without compromising sensitivity, specificity, dynamic range, or PCR efficiency. 

GeneAll has engineered most optimal reaction buffers to minimize non-specific amplification and / or primer dimer formation.

RealAmp™ 2X qPCR Master mix, supplied in a 2X concentration, is a ready-to-use master mix to perform real-time PCR using dsDNA binding fluorescent dye.

01

High Sensitivity

  • gDNA : 10 pg~100 ng
  • cDNA : 1 pg~100 ng
02

High Specificity

  • Optimized formulation of the conventional dsDNA binding fluorescent dye-based real-time PCR
  • Significantly suppress primer dimers and nonspecific amplification
03

Easy of use

  • Convenient 2X master mix format
  • Optional : 2 reference dye (Low ROX / High ROX)
04

APPLICATIONS

  • Suitable for real-time PCR amplification using dsDNA binding fluorescent dye.

specifications

Technical Information

Features Specifications
Optimal amplification size 80~150 bp (Do not over 300 bp)
Storage condition Stable for 1 year at -20°C
Keep the kit in dark place
Do not repeat freeze and thaw cycle.

Kit Contents

RealAmp™ 2X qPCR Master Mix

with Low ROX

801-020

(200 reactions)

801-050

(500 reactions)

2X qPCR Master Mix 1 ml × 2 tubes 1 ml × 5 tubes
Low Rox reference dye 0.2 ml × 1 tube 0.5 ml × 1 tube

RealAmp™ 2X qPCR Master Mix

with High ROX

801-021

(200 reactions)

801-051

(500 reactions)

2X qPCR Master Mix 1 ml × 2 tubes 1 ml × 5 tubes
High Rox reference dye 0.2 ml × 1 tube 0.5 ml × 1 tube

Protocols

Supplementary Protocols

Resources

Application Notes

References

Brochures