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AmpONE™ 2X Taq Premix

AmpONE™ 2X Taq Premix is purified from the cloned Thermus aquaticus DNA polymerase gene in Escherichia coli (E.coli). This premix contains all reaction components required for conventional PCR, such as reaction buffer, dNTP, loading dye, stabilizer and sediment in addition to Taq DNA polymerase. It is recommended for use in conventional PCR (below 5 kb), TA cloning and colony PCR. It is ready-to-use mixture pipetting steps are minimized, reducing the possibility of errors and contamination.

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Overview

AmpONE™ 2X Taq Premix is purified from the cloned Thermus aquaticus DNA polymerase gene in Escherichia coli (E.coli).

This premix contains all reaction components required for conventional PCR, such as reaction buffer, dNTP, loading dye, stabilizer and sediment in addition to Taq DNA polymerase. 

It is recommended for use in conventional PCR (below 5 kb), TA cloning and colony PCR.

It is ready-to-use mixture pipetting steps are minimized, reducing the possibility of errors and contamination.

01

Description

  • This premix contains all reaction components required for conventional PCR, such as reaction buffer, dNTP, loading dye, stabilizer and sediment in addition Taq DNA polymerase.
02

Ready to use

  • Pre-aliquoted in 8-strip tube
  • Suitable for the amplification of fragments up to 5 kb from template.
03

3' end A-tailing

  • 5’→3’ polymerase activity
  • No 3’→5’ exonuclease (proofreading) activity
04

Applications

  • Suitable for Conventional PCR (up to 5 kb), TA cloning, Colony PCR, High through-put PCR, Routine PCR requiring high reproducibility, DNA sequencing template preparation.

specifications

Technical Information

Features Specifications
Synthesis efficiency ~5 kb
Exonuclease activity None detected
Endonuclease activity None detected
Storage condition Stable for 18 months at -20°C

Kit Contents

Cat. No. 526-200 (Reaction vol. 20 μl)

12 x 8-strip PCR tube with PCR tube rack

Technical Data

 

Protocols

Supplementary Protocols