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Riboclear™

GeneAll® Riboclear™ (Plus) provides an easy and rapid method for RNA cleanup or concentration from various RNA samples in just 6 minutes. Riboclear™ series eliminates alcohol precipitation by binding of RNA with column, allowing rapid and convenient preparation from various samples simultaneously.

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Overview

Riboclear™ (Plus) provides an easy and rapid method for RNA cleanup or concentration from various RNA samples in just 6 minutes.

Purified RNA with Riboclear™ series are free of salts and enzymes in yields reaching 95% and 

are suitable for dot blotting, in vitro translation, cloning, RT-PCR, RNase protection assays and other analytical procedures. 

Riboclear™ Plus provides DNase I for removal of DNA and micro column for concentration of total RNA.

01

Format

Riboclear™ (Plus) utilizes glassfiber membrane technology for RNA cleanup or concentration.

  • Spin column format
02

Stable and consistent yield

  • Sample size : ~100 μl
  • High recovery rate : ~95%
  • Riboclear™ Plus provides DNase I for removal of DNA and micro column for concentration of total RNA.
03

Simple and safe procedure

  • Preparation time
    : ~6 minutes / ~17 minutes (Plus)
  • No use of organic solvents
  • No alcohol precipitation
04

Applications

  • Ready for use in RT-PCR, northern blotting, dot blotting, in vitro translation, molecular cloning, real-time PCR, RNase protection assays and other analytical procedures.

specifications

Technical Information

Features Riboclear™  Riboclear™ Plus
Format Column Type F with collection tubeColumn Type S (Micro) with collection tube
Sample size ~100 μl ~100 μl
RNA recovery rate ~95%~95%
Maximum loading volume ~750 μl ~800 μl
Minimum elution volume ~30 μl ~20 μl
Binding capacity ~500 μg ~100 μg
Preparation time ~6 minutes~17 minutes

Kit Contents

Riboclear™

(Cat. No. 303-150)

Riboclear™ Plus

(Cat. No. 313-150)

  • Column type F
  • Collection tubes
  • Buffer MS (concentrate)
  • Buffer RNW (concentrate)
  • Nuclease-free water
  • Micro column type S
  • Collection tubes
  • Buffer MS (concentrate)
  • Buffer RNW (concentrate)
  • Nuclease free water
  • DNase I
  • DNase I buffer
  • Technical Data

        Reproducibility Test

                     

        Downstream Application Test

                     

    Protocols

    Supplementary Protocols