GeneAll® Ribospin™ II is devised to purify RNA from cultured cells or animal tissues. With the GeneAll’s glassfiber membrane technology, highly pure RNA can be conveniently isolated in less than 30 minutes instead of the time consuming and hazardous conventional methods which require alcohol precipitation or toxic chemicals such as phenol / chloroform. The optimized buffer system of Ribospin™ II maximizes the specific binding efficiency of RNA to the glassfiber membrane but minimizes the contamination of impurities by a series of optimized wash buffer. Also, the contaminated DNA residues can be easily eliminated during the preparation by on-column digestion using DNase I included in this kit.
GeneAll® Ribospin™ II is devised to purify RNA from cultured cells or animal tissues.
With the GeneAll’s glassfiber membrane technology, highly pure RNA can be conveniently isolated in less than 30 minutes instead of the time
consuming and hazardous conventional methods which require alcohol precipitation or toxic chemicals such as phenol / chloroform.
The optimized buffer system of Ribospin™ II maximizes the specific binding efficiency of RNA to the glassfiber
membrane but minimizes the contamination of impurities by a series of optimized wash buffer. Also, the contaminated DNA
residues can be easily eliminated during the preparation by on-column digestion using DNase I included in this kit.
Ribospin™ II utilizes glassfiber membrane technology for the isolation of total RNA.
Features | Ribospin™ II | ||
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Format | Column Type F with collection tube | ||
Maximum amount of starting samples | ~30 mg tissue or ~1 x 107 cells | ||
Maximum loading volume | ~750 μl | ||
Minimum elution volume | ~30 μl | ||
Binding capacity | ~500 μg | ||
Kit Contents
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Total RNA Isolation from CHO and rat liver cell Using RibospinTM II
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Comparison Data - Real time PCR amplificaton and cDNA synthesis. |