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DirEx™ Fast

GeneAll® DirEx™ Fast, a premixed format which contains all reaction reagents in 8-strip tube is designed for the easy and simple preparation of template DNA in PCR applications. The whole procedure can be completed in a single tube in just 8 minutes and is composed of two steps, the incubation and the inactivation. It is basically designed to use PCR thermal cycler for whole procedure, although the conventional bath can be employed. The simple procedure of DirEx™ Fast requires neither the centrifuge step nor the additional pipetting, and it facilitates the multiple preparations from many samples. Simultaneous preparation from many samples with minimum handling will help guarantee the fidelity of the analysis. DirEx™ Fast can be used for the preparation of template DNA from a wide range of biological and forensic samples, such as mammalian blood, hairs, tissues, swabs, blood stains, cigarette butts and cultured cells. Prepared DNA can be applied directly to PCR applications and / or stored in a freezer for storage.

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Overview

GeneAll® DirEx™ Fast, a premixed format which contains all reaction reagents in 8-strip tube is designed for the easy and simple preparation of template DNA in PCR applications. The whole procedure can be completed in a single tube in just 8 minutes and is composed of two steps, the incubation and the inactivation. 

It is basically designed to use PCR thermal cycler for whole procedure, although the conventional bath can be employed. 

The simple procedure of DirEx™ Fast requires neither the centrifuge step nor the additional pipetting, and it facilitates the multiple preparations 

from many samples. Simultaneous preparation from many samples with minimum handling will help guarantee the fidelity of the analysis.

DirEx™ Fast can be used for the preparation of template DNA from a wide range of biological and forensic samples, such as mammalian blood, hairs, tissues, swabs, blood stains, cigarette butts and cultured cells. Prepared DNA can be applied directly to PCR applications and / or stored in a freezer for storage.

01

Format

DirEx™ Fast (premixed format which contains all reaction reagents in 8-strip tube) 

  • Specially formulated buffer system
  • DirExTM Fast is basically designed to use PCR thermal cycler for whole procedure.
02

Simple and easy procedure

  • Completed in a single tube and it takes just 8 minutes
  • No requirement of the centrifuge step or the additional pipetting.
03

Safe procedure

  • No phenol/chloroform extraction
  • No organic extraction
04

Applications

  • Can be used for the preparation of template DNA from a wide range of biological and forensic samples, such as mammalian blood, hairs, tissues, swabs, blood stains, cigarette butts and cultured cells.
  • Suitable for PCR and real-time PCR applications.

specifications

Technical Information

Features DirEx™ Fast
Format
    Specially formulated buffer system
Amount of starting samples20 μl whole blood
2 x 106 cultured cells
10 mg animal tissue
Preparation time 8 minutes

Kit Contents

DirEx™ Fast 

  • Cat. No. 260-011 for Tissue
  • Cat. No. 260-021 for Cultured cell
  • Cat. No. 260-031 for Whole blood
  • Cat. No. 260-041 for Blood stain
  • Cat. No. 260-051 for Hair
  • Cat. No. 260-061 for Buccal swab
  • Cat. No. 260-071 for Cigarette
    		•
  • 96 tubes (0.2 μl 8-tube strip x 12 ea)
  • Should be stored at -20°C

    • Technical Data

        Procedures of DirExTM Fast

         PCR Amplification Test with extracted DNA by DirExTM Fast

           

    Protocols

    Handbooks

    Supplementary Protocols