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Hybrid-R™ Blood RNA

GeneAll® Hybrid-R™ Blood RNA is a complete kit with ready-to-use reagent for the isolation of total RNA from up to 0.25 ml whole blood sample. This kit utilizes the lysis method of RiboEx™ LS which has a powerful ability of cell-lysis and the purification method based on glassfiber membrane technology. Fast and convenient procedure of Hybrid-R™ Blood RNA takes only 30 minutes for complete preparation of pure RNA.

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Overview

GeneAll® Hybrid-R™ Blood RNA is a complete kit with ready-to-use reagent for the isolation of total RNA from up to 0.25 ml whole blood sample. 

This kit utilizes the lysis method of RiboEx™ LS which has a powerful ability of cell-lysis and 

the purification method based on glassfiber membrane technology. 

Fast and convenient procedure of Hybrid-R™ Blood RNA takes only 30 minutes for complete preparation of pure RNA.

01

Format

Hybrid-R™ Blood RNA utilizes the lysis method of RiboExTM LS which has a powerful ability of lysis and the purification method based on glassfiber membrane technology. 

  • Combined Sample lysis by RiboExTM LS with Spin column format
02

Stable and consistent yield

  • Maximum yield : 3 μg per 0.25 ml whole blood
  • No genomic DNA contamination
03

Simple and safe procedure

  • Preparation time : ~30 minutes
  • No ethanol precipitation
04

Applications

  • Suitable for the isolation of Poly A+ RNA, northern blotting, dot blotting, in vitro translation, cloning, RT-PCR, RPA and other analytical procedures

specifications

Technical Information

Features Hybrid-R™ Blood
Format Column Type W with collection tube
Maximum amount of starting samples

250 μl 

Minimum amount of starting samples

100 μl

Maximum loading volume ~700 μl
Minimum elution volume ~30 μl
Maximum Binding capacity ~100 μg

Kit Contents

Hybrid-R™ Blood RNA (Cat. No. 315-150)

  • RiboExTM LS
  • Column Type W (mini)
  • EzPureTM Filter 
  • Collection tubes
  • Buffer RB1 (concentrate)
  • Buffer RBW (concentrate)
  • Buffer RNW (concentrate)
  • Nuclease-free water
  • Technical Data

        Comparison Data - Total RNA Purification Using Hybrid-RTM Blood

           

        Verification of Genomic DNA Contamination and RT-PCR Result

           
             

    Protocols

    Supplementary Protocols