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Exgene™ Blood SV

GeneAll® Exgene™ Blood SV provides a simple and rapid method for the isolation of total DNA from fresh or frozen whole blood, serum, buffy coat, plasma, virus and cultured cells. Purification procedure is so simple and optimized to simultaneous processing of multiple samples. Exgene™ Blood SV yields pure DNA ready for direct PCR in just 20 minutes (mini) and 1 hour (Midi / MAXI).

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Overview

GeneAll® Exgene™ Blood SV provides a simple and rapid method for the isolation of total DNA from fresh or frozen whole blood, serum, 

buffy coat, plasma, virus and cultured cells. Purification procedure is so simple and optimized to simultaneous processing of multiple samples. 

Exgene™ Blood SV yields pure DNA ready for direct PCR in just 20 minutes (mini) and 1 hour (Midi / MAXI).

01

Format

Exgene™ Blood SV utilizes glass microfiber membrane.

  • Spin column format
  • Midi and MAXI kits require the centrifuge which has swing bucket rotor and ability of 4,000 x g at least.
02

High purity

  • Stable and consistent result
  • High yield and purity : A260/A280 = 1.8~2.0
03

Simple and safe procedure

  • No phenol/chloroform extractions
  • No alcohol precipitation
04

Applications

  • Ready for use in PCR, Southern blotting, AFLP, RFLP, RAPD and other enzymatic reactions.

specifications

Technical Information

Features Blood SV mini Blood SV Midi Blood SV MAXI
Format Column type G (mini) with collection tubeColumn type G (Midi) with collection tubeColumn type G (MAXI) with collection tube
Maximum sample size~200 μl1~2 ml3~10 ml
Preparation time20~30 minutes

40~55 minutes 

40~55 minutes 

Typical yield4~20 μg20~80 μg80~400 μg
Elution volume30~400 μl200~600 μl0.4~2 ml

Kit Contents

Blood SV mini

(Cat. No. 105-101 / 105-152)

Blood SV Midi

(Cat. No. 105-226 / 105-201)

Blood SV MAXI

(Cat. No. 105-310 / 105-326)
  • mini column type G
  • Collection tubes
  • Buffer BL
  • Buffer BW (concentrate)
  • Buffer TW (concentrate)
  • Buffer AE
  • Proteinase K
  • PK Storage buffer
    		•
  • Midi column type G
  • Collection tubes
  • Buffer BL
  • Buffer BW (concentrate)
  • Buffer TW (concentrate)
  • Buffer AE
  • Proteinase K
  • PK Storage buffer
    		•
  • MAXI column type G
  • Collection tubes
  • Buffer BL
  • Buffer BW (concentrate)
  • Buffer TW (concentrate)
  • Buffer AE
  • Proteinase K
  • PK Storage buffer
    		•

    Technical Data

        Genomic DNA Extraction from Various Samples

           

        Compatibility Test with Restriction Enzymes

           

        PCR Amplification Test