Endotoxins (lipopolysaccharides, LPS) are present in the cell membrane of gram-negative bacteria, such as Escherichia coli. It is a common contaminant in plasmid preparations and can significantly reduce transfection efficiencies, if not removed during DNA preparations. GeneAll® Exfection™ Plasmid EF provides simple and fast method for the purification of plasmid DNA with low endotoxin contaminants.
Endotoxins (lipopolysaccharides, LPS) are present in the cell membrane of gram-negative bacteria, such as Escherichia coli.
It is a common contaminant in plasmid preparations and can significantly reduce transfection efficiencies, if not removed during DNA preparations.
GeneAll® Exfection™ Plasmid EF provides simple and fast method for the purification of plasmid DNA with low endotoxin contaminants.
Exfection™ Plasmid EF utilizes glass microfiber membrane.
Features | Plasmid EF Midi | ||
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Format | Column Type E with collection tube | ||
Recommended sample volume | 30~150 ml LB | ||
Lysate clearing | EzClear™ Filter | ||
Preparation time | under 70 minutes | ||
Maximum loading volume | 15 ml | ||
Binding capacity | 300 μg | ||
Endotoxin level | <0.1 EU/μg | ||
Maximum Elution volume | 500 μl | ||
Kit Contents
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Transfection Efficiency
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