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Hybrid-Q™ Plasmid Rapidprep

Hybrid-Q™ Plasmid Rapidprep provides two methods for easy and rapid preparation of plasmid DNA from the mini scale bacterial cells. Plasmid DNA can be prepared from up to 10 ml of overnight culture by conventional miniprep method with standard protocol. Alternatively, up to 3 ml of sample can be processed by rapid protocol in just 10 min with new patented EzClear™ Filter and simultaneous processing of multiple samples can be easily performed.

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Overview

Hybrid-Q™ Plasmid Rapidprep provides two methods for easy and rapid preparation of plasmid DNA from the mini scale bacterial cells. 

Plasmid DNA can be prepared from up to 10 ml of overnight culture by conventional miniprep method with standard protocol. 

Alternatively, up to 3 ml of sample can be processed by rapid protocol in just 10 min with new patented EzClear™ Filter and 

simultaneous processing of multiple samples can be easily performed.

01

Format

Hybrid-Q™ Plasmid Rapidprep utilizes glassfiber membrane technology for easy and rapid preparation of plasmid DNA.

  • Spin column format
  • EzClear™ Filter is assembled with GeneAll® spin column, and this column stack makes it one-step the clearance of lysate and the binding of plasmid DNA to spin column membrane.
02

Stable and consistent yield

  • High recovery rate : 85~95%
  • Binding capacity : 30 μg
03

Simple and safe procedure

  • Preparation time
    : just 10 min with new patented EzClearTM Filter
  • No use of organic solvents
  • No alcohol precipitation
04

Applications

  • Ready for use in PCR, cloning, fluorescent sequencing, synthesis of labeled hybridization probes, cell transfection, electroporation and enzymatic restriction analysis without further manipulation.

specifications

Technical Information

Features Hybrid-Q™ 
Format Column Type Q with collection tube
EzClearTM Filter with collection tube
Recommended sample volume (High copy) 2~5 ml
Maximum sample volume (Low copy) 10 ml
Maximum loading volume of EzClearTM Filter ~600 μl
Maximum loading volume of spin column ~800 μl
Minimum elution volume ~40 μl
Binding capacity ~30 μg

Kit Contents

Hybrid-Q™ (Cat. No. 100-150 / 100-102)

  • Column type Q 
  • EzClearTM Filter
  • Collection tubes
  • Buffer S1
  • Buffer S2
  • Buffer S3
  • Buffer AW (concentrate)
  • Buffer PW (concentrate)
  • Buffer EB
  • RNase A (20 mg/ml)
  • Technical Data

        DNA Automated Sequencing Analysis

             

        Stable and Reproducible Result

             

        Compatibility Test with Restriction Enzymes

           

    Protocols

    Supplementary Protocols