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LARGE SCALE CONDITIONING FOR HIGH THROUGHPUT SERVICES
1 - ORDERING
For ordering please contact us at : oligos@generalbiosystem.com to receive the high throughput order form which should be attached to your e-mail order.
2 - PLATE FORMATS
A) FORMATS
Microtube plate
- 96 well plate, individual 1ml capacity removable tubes capped
in strips of 8.
Deepwell plate - 96 well plate.1ml capacity wells. Adhesive
sealing.
Microtitration plate - standard 96 well plate. 200 µl
capacity wells.
Thermosealed with aluminium foil.
B) CONDITIONING
|
Forward
Master Plate
& Reverse Master Plate |
Forward
Dilute Plate
& Reverse Dilute Plate |
Mixed
Forward
& Reverse Plate |
 |
+
|
+ |
+ |
 |
+
|
+ |
+ |
 |
+
|
+ |
+ |
C) ADJUSTMENT
Oligos in each of the plates can be adjusted to your desired
final concentration as outlined below:
| Final
Concentrations (FINCO) available in µM or µg/ml |
| Quantity
(OD) |
2 |
5 |
10 |
| Concentration
range (µM) |
20-200 |
30-400 |
100-800 |
| Concentration
range (µg/µl) |
0.2
- 1 |
0.3
– 3 |
0.75
– 6 |
For higher quantities,
please contact us.
D) DISTRIBUTION
The oligos are dispensed as follows in the microwell plates:
1st
oligo |
9th
oligo |
17th
oligo |
25th
oligo |
33th
oligo |
41th
oligo |
49th
oligo |
57th
oligo |
65th
oligo |
73th
oligo |
81th
oligo |
89th
oligo |
| A1 |
A2 |
A3 |
A4 |
A5 |
A6 |
A7 |
A8 |
A9 |
A10 |
A11 |
A12 |
| B1 |
B2 |
B3 |
B4 |
B5 |
B6 |
B7 |
B8 |
B9 |
B10 |
B11 |
B12 |
| C1 |
C2 |
C3 |
C4 |
C5 |
C6 |
C7 |
C8 |
C9 |
C10 |
C11 |
C12 |
| D1 |
D2 |
D3 |
D4 |
D5 |
D6 |
D7 |
D8 |
D9 |
D10 |
D11 |
D12 |
| E1 |
E2 |
E3 |
E4 |
E5 |
E6 |
E7 |
E8 |
E9 |
E10 |
E11 |
E12 |
| F1 |
F2 |
F3 |
F4 |
F5 |
F6 |
F7 |
F8 |
F9 |
F10 |
F11 |
F12 |
| G1 |
G2 |
G3 |
G4 |
G5 |
G6 |
G7 |
G8 |
G9 |
G10 |
G11 |
G12 |
| H1 |
H2 |
H3 |
H4 |
H5 |
H6 |
H7 |
H8 |
H9 |
H10 |
H11 |
H12 |
3 - SERVICES
A)
ALIQUOTTING
The oligos from the above plates can be aliquotted into multiple plates (amount of aliquots limited by final volume in each plate). Please indicate number of aliquots required for each type of plate (Forward, Reverse, Mixed etc.).
B)
DOCUMENTATION
An Oligonucleotide Technical Data Sheet is shipped with all
orders and contains all the information related to the oligos
we manufactured for you including sequence, quantity, epsilon,
molecular mass, concentration and Tm. The plate identification
number and the position of each oligo on the master plates (A1
– H12) are also indicated. The data sheet is sent as a
hard copy with your order. You can also request a copy by e-mail.
C)
LABELLING OF PLATES AND CUSTOM BARCODING
All our plates are labelled by default with a Genset barcode and plate identification number to allow you to cross-reference your plate with the information on the Oligonucleotide Technical Data Sheet.
We can also create custom barcodes to enable you to have instant access to your own information on each oligo order based on the information you give us. Please contact us for further details of this service.
D)
DELIVERY TIME
| Number
of 96-well plates (wells or tubes) |
1
to 5 |
5
to 10 |
10
to 20 |
| Turn
around time of synthesis, deprotection & desalting |
3
days |
4
days |
5
days |
| Concentration
adjustment |
+
6 h |
+
12 h |
+
24 h |
| Mixed
plate preparation |
+
12 h |
+
1 day |
+
2 days |
| Aliquotting |
+
1 day |
+
2 days |
+
4 days |
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