| | Truly positive |
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| | Improve the allele-specific PCR method of SNP genotypingFixed price per primer (7 to 40 mers)
TrueSNPTM allele-specific PCR primers are high-specificity primers designed to improve the allele-specific PCR (AS PCR) method of SNP genotyping. TrueSNP AS PCR primers significantly reduce the erroneous amplifications of mismatched DNA allele-specific primers, thus enabling a researcher to reliably detect a potential SNP using this method.
For your convenience,
TrueSNP AS PCR primers are available at a fixed price per primer (7 to 40 mers).
TrueSNP AS PCR primers feature a single, high-affinity, high-specificity Locked Nucleic AcidTM; (LNATM) residue placed at the 3'-end of the primer (in the SNP query position), conferring increased mismatch discrimination. All other bases are DNA
eg. ACGTACAG+T (+ denotes the LNA base).
- Guaranteed yield of at least 5 OD (approx. 25 nmols*)
- Deprotected and desalted
- Delivered in de-ionized water at a concentration of 100 uM
- Shipped within 3 to 4 working days of receiving your order, pending successful QC validation
Specifications of TrueSNP AS PCR Primers
| Quality control | All our oligos undergo vigorous process monitoring and strict quality control including OD measurement, concentration calculation and PAGE analysis. |
| Purification | Fully deprotected and desalted |
| Guaranteed yield | At least 5 OD (approx. 25 nmols*) |
| Length | 7 to 40 mers |
| Bases | LNA (+A, +C, +G or +T) (+ denotes the LNA base)
DNA (A,C, G or T) inosine, wobbles (degenerate bases) |
| Modifications | None |
| Backbone | Phosphodiester bond |
| Format | Delivered in de-ionized water at a concentration of 100 uM |
| Turn-around time (TAT) | Shipped within 3 to 4 working days of receiving your order, pending successful QC validation |
| Storage and stability | Although oligonucleotides are stable in solution at 4℃ for up to 2 weeks, Proligo recommends storage should be at -20℃. Repetitive freeze-thaw cycles should be avoided by storing as aliquots. Storing at concentrations above 20 uM is recommended. Proligo guarantees its oligonucleotides for 6 months, stored under the above conditions. |
| Shipment | Shipped by express delivery in solution in individual, transparent tubes |
Oligonucleotide
Technical Data Sheet | Oligonucleotides are delivered with an Oligonucleotide Technical Data Sheet, which includes oligo name, sequence, concentration, precise quantity in OD and nmols, Tm, MW, size, extinction coefficient and purification data. |
| Pricing | Please contact your local Proligo representative |
| Ordering | On-line or by email |
| *Estimate 1 OD = 5 nmols = 30ug, for a 20 mer oligo |
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