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Length
GC content %
Molecular Weight: g/mol
Melting Temperature (Tm) °C
Epsilon 260 nm
Quantity of 1 OD is equal to nmoles µg
How Proligo calculates the Tm of your oligos

For oligonucleotides <= 20 bases, we use the classical Tm = 2(A + T) + 4(G + C) calculation
for melting temperature, according to Wallace rule for short oligonuceotides (1).

For oligonucleotide > 20 bases, calculation is based on the Nearest-Neighbor formula
with [salt] = 50 mM and single strand concentration = 250 pM (2).

References

 (1) Wallace, R.B.; Shaffer, J.; Murphy R.F.; Bonner, J.; Hirose, T.; Itakura, K.; (1979)
      Nucelic Acid Res. 6, 3543
 (2) Breslauer, K.J.; Frank, R.; Blöcker, H.; Marky, L.A.; (1986)
      Proc.Natl.Acad.Sci.USA 83, 3746-3750