Plasmid DNA Purification
 Fragment DNA Purification
 Genomic DNA Purification
 RNA Purification
 Protein Purification
 Automatic NA Purification
 Others
Plasmid DNA Purification

Product ExfectionTM Plasmid LE / EF
Brand GeneAll®
Storage Room temperature (15~25℃)
Cat.No.   Product Description Size Cart/Quote/Request
111-150 ExfectionTM Plasmid LE, mini 50      
111-102 ExfectionTM Plasmid LE, mini 200      
111-226 ExfectionTM Plasmid LE, Midi 26      
111-201 ExfectionTM Plasmid LE, Midi 100      
121-220 ExfectionTM Plasmid EF, Midi 20      
121-201 ExfectionTM Plasmid EF, Midi 100      

 

Description

ExfectionTM plasmid LE (Low Endotoxin) and EF(Endotoxin-Free) provide simple and fast method for the
purification of plasmid DNA with low endotoxin contaminants.Endotoxins (also known as lipopolysaccharides, LPS) are present in the cell membrane of gram-negative bacteria, such as Escherichia coli.
It is a common contaminant in plasmid preparations and can significantly reduce transfection efficiencies, if not removed during DNA preparations. These kits use two methods for the removal of endotoxins: advanced phase separation and endotoxin removal washing. Endotoxin levels can be reduced to 0.1 EU/㎍ with ExfectionTM EF and to 10 EU/㎍ with ExfectionTM LE. Prepared plasmid DNA can be used for the transfection of most of cell lines in addition to most of molecular biological applications.

 

Features and Benefits

■ Spin column format based on glassfiber membrane
■ Convenient clearing of lysate with EzClearTM filter (Midi)
■ High plasmid recoveries with high purity
■ Fast preparation time and simple procedure
■ High transfection efficiency in most cell-lines
■ No need of additional materials
■ No use of organic solvents

 

Procedure


 

Specification