ExfectionTM plasmid LE (Low Endotoxin) and EF(Endotoxin-Free) provide simple and fast method for the
purification of plasmid DNA with low endotoxin contaminants.Endotoxins (also known as lipopolysaccharides, LPS) are present in the cell membrane of gram-negative bacteria, such as Escherichia coli.
It is a common contaminant in plasmid preparations and can significantly reduce transfection efficiencies, if not removed during DNA preparations. These kits use two methods for the removal of endotoxins: advanced phase separation and endotoxin removal washing. Endotoxin levels can be reduced to 0.1 EU/㎍ with ExfectionTM EF and to 10 EU/㎍ with ExfectionTM LE. Prepared plasmid DNA can be used for the transfection of most of cell lines in addition to most of molecular biological applications.
Features and Benefits
■ Spin column format based on glassfiber membrane
■ Convenient clearing of lysate with EzClearTM filter (Midi)
■ High plasmid recoveries with high purity
■ Fast preparation time and simple procedure
■ High transfection efficiency in most cell-lines
■ No need of additional materials
■ No use of organic solvents